Wet-Laboratory Coordinator
My passion lies in teaching and learning! Being a laboratory coordinator, the development of wet lab practices in the fields of Biological and Health Sciences has been the cornerstone of my professional engagement. These practices are aimed to provide hands-on training to undergraduate learners in various academic programs and expand their scientific skillset for a successful career.
I have the technical and pedagogical expertise in creating resources such as videos, presentations, manuals, quizzes, etc., and supervising teaching assistants on facilitating laboratory workshops, both in-person and virtually through online learning platforms. With a Ph.D. in Biotechnology from Brock University (Ontario, Canada), I have spent the last ten years of my life learning and teaching a diverse range of biomolecular tools, biochemical and cellular/immunological assays, microbiological techniques, and physiological experiments.
Cell Culture: Bacterial cells, mammalian cell lines (lung carcinoma models: H460 and A549, and breast cancer models: MCF7 and 4T1), & insect cell lines (Sf9 and Sf21).
Molecular Cloning: DNA isolation and purification from bacterial and mammalian cells, PCR, restriction digestion, bacterial transformation, antibiotic and blue-white selection, & plasmid miniprep; viral plaque assay & heterologous protein expression using Baculovirus expression vector system; gel electrophoresis: agarose, SDS-PAGE, & native-PAGE.
Immunochemistry: Blood typing, complement activation assay, Western blot, ELISA, histochemistry, & β-hexosaminidase assay.
Cellular Imaging: Phase-contrast, fluorescent, & confocal microscopy.
Human Physiology: Electrophysiology – study of stimulus-response of skeletal muscles and nerves; respiratory physiology – study of ventilation parameters by spirometry; cardiac physiology; & endocrinology – study of insulin and glucagon in the regulation of glucose metabolism & study of hCG as an indicator of human pregnancy.
Protein Chromatography: IMAC, FPLC, SEC, & IEX.
Surface Chemistry: SPR, DPI, & FRET-based liposomal assays.
Positions:
- Wet-Laboratory Coordinator, Department of Health Sciences, Brock University.
- Senior Lab Demonstrator, Department of Biological Sciences, Brock University.
- Science Stores Assistant, Faculty of Mathematics and Sciences, Brock University.
- Lab Demonstrator, Departments of Biologicals Sciences, Chemistry, and Health Sciences, Brock University.
- Examination Invigilator, Student Accessibility Services, Brock University.
Academic Services:
- Member, Academic Safety Committee (ASC), Brock University.
- Project Developer, Scientifically Yours (SY), Faculty of Applied Health Sciences, Brock University.
Certifications:
- Transportation of Dangerous Goods (TDG) Training, Health, Safety & Wellness (HSW), Brock University.
- Graduate Teaching Assistant (GTA) Practicum, Centre for Pedagogical Innovation (CPI), Brock University.
Awards/Recognition:
- Distinguished Graduate Student Award – Biotechnology, Brock University.
- Teaching Assistant Award, Brock University.
- CSMB Travel Award, Canadian Society for Molecular Biosciences (CSMB), 58th Annual Conference, Halifax, NS, Canada.
- The Dean of Graduate Studies Spring Research Fellowship (Doctoral), Brock University.
- The Dean of Graduate Studies Spring Research Fellowship (Masters), Brock University.
Mukherjee, P., Madarati, H., Ridgway, N. D., & Atkinson, J. (2018). Lipid and membrane recognition by the oxysterol binding protein and its phosphomimetic mutant using dual polarization interferometry. Biochimica et biophysica acta. Biomembranes, 1860(11), 2356–2365. https://doi.org/10.1016/j.bbamem.2018.05.022
Coordinated and demonstrated laboratory sessions in the courses listed below.
CURRENT COURSES
Health Sciences:
HLSC 2P09 – Human Physiology
HLSC 3P02 – Human Immunology
HLSC 4P02 – Medical Immunology
HLSC 4P95 – Human Pathology
HLSC 4P97 – Tropical Parasites of Humans and Animals
COURSES IN PAST
Biological Sciences:
BCHM 3P01 – Metabolic Biochemistry
BIOL 2P02 – Introduction to Molecular Biology (formerly BIOL 2F01 – Principles of Molecular and Cellular Biology)
BIOL 2Q04 – Principles of Ecology
Biotechnology:
BTEC 3P09 – Bioreactor Processes
