Structural Parameters of Aqueous Phospholipid Mixtures

Membrane Biophysics Laboratory

R. P. Rand

Osmotic Pressure Data

Structural Parameters of Aqueous phospholipid Mixtures

Explanation
and Calculation
of Structural Parameters

Lamellar Phases

Hexagonal Phases

Lamellar Phases

PC PE charged/others Mixtures
egg PC eggPE DHDAA DOPE/DOPC 3/1
DLPC egg PE-trans DOPS DGDG/SOPC 44/55
DMPC-27 MMPE DGDG DGDG/POPE 1/1
DPPC-25 DMPE   POPE/SOPC 19/1
DPPC-50 POPE   POPE/SOPC 9/1
DOPC     POPE/SOPC 4/1
DSPC     POPE/SOPC 2/1
16-22PC     POPE/SOPC 3/2
SOPC     POPE/SOPC 1/1
      egg PC/chol 1/1
      DPPC/chol 1/1
      DPPC/chol 8/1
      egg PC/DAG 12.5%


Phosphatidylcholines

Parameters

units of Angstroms unless otherwise indicated

eggPC DLPC DMPC-27 DPPC-25 DPPC-50 DOPC DSPC SOPC 16,22PC
References
 
Parsegian, V. A.
et.al.
PNAS 76:2750
Lis, L. J. et.al. Biophys. J. 37:657and667
Rand, R. P. et.al. Chemica Scripta 25:15
Rand, R. P. and Parsegian, V. A. BBA 988:351
d repeat
 
61.9
59
62.2
63.8
67
64
67.3
64.6
63.5
equilibrium
concentration
0.60
0.54
0.57
0.74
0.54
0.56
0.71
0.63
0.60
molecular
surface area
69.5
64
61.7
48.6
68.1
72.1
51.6
64.3
69.3
lipid bilayer
thickness
37
31.6
35.7
47.1
35.9
35.9
47.7
40.6
38.3
water layer
thickness
24.9
27.4
26.5
16.7
31.1
28.1
19.6
24
25.2
hydrocarbon
layer thickness
25.9
18.9
23.1
30.4
24.1
25.4
31.9
28.7
27.4
polar group
thickness
11.1
12.7
12.6
16.7
11.8
10.5
15.8
11.9
10.9
water volume
per lipid
866
877
816
405
1059
1013
506
771
873
lambda
 
2.1
2.0
2.2
1.2
2.1
2.1
1.3
2.0
2.1
Po (dyne/cm2)
 
10.6
10.6
10.5
12.3
11
10.6
12.9
10.5
10.1
G min (erg/cm2)
 
0.03
0.01
0..02
0.03
0.01
0.01
0.15
0.02
0.01
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Phosphatidylethanolamines

Parameters

units of Angstroms unless otherwise indicated

eggPE eggPE-trans MMPE DMPE POPE
References
 
Rand, R. P. and Parsegian, V. A. in Molecular mechanisms of membrane fusion.
Rand, R. P. and Parsegian, V. A. BBA 988:351
d repeat
 
52.9
52
61.8
63.1
53.2
equilibrium
concentration
0.64
0.72
0.66
0.64
0.79
molecular
surface area
72.1
65
60.7
62.6
56.6
lipid bilayer
thickness
33.8
37.4
40.8
40.4
41.8
water layer
thickness
19.1
14.6
21
22.7
11.4
hydrocarbon
layer thickness
25.0
27.7
29.6
28.8
28.7
polar group
thickness
8.8
9.7
11.2
11.6
11.4
water volume
per lipid
690
474
637
713
323
lambda
 
1.32
1.08
1.76
1.83
0.82
Po (dyne/cm2)
 
12.5
12.3
10.3
10.4
12.5
G min (erg/cm2)
 
0.140
0.196
0..13
0.011
0.161
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Other lipids

Parameters

units of Angstroms unless otherwise indicated

DHDAA DOPS DGDG
References
 
Rand, R. P. and Parsegian, V. A. in Molecular mechanisms of membrane fusion.
Rand, R. P. and Parsegian, V. A. BBA 988:351
d repeat
 
 
53.5
53.2
equilibrium
concentration
 
0.74
0.73
molecular
surface area
 
70
79.8
lipid bilayer
thickness
 
39.6
38.8
water layer
thickness
 
13.9
14.4
hydrocarbon
layer thickness
 
26.2
22.5
polar group
thickness
 
13.4
16.3
water volume
per lipid
 
485
574
lambda
 
 
 
1.7
Po (dyne/cm2)
 
 
 
10.3
G min (erg/cm2)
 
 
 
0.24
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Mixtures

Parameters

units of Angstroms unless otherwise indicated

DOPE/DOPC 3/1 POPE/SOPC 19/1 POPE/SOPC 9/1 POPE/SOPC 4/1 POPE/SOPC 2/1 POPE/SOPC 3/2 POPE/SOPC 1/1
References
 
Rand, R. P.
et.al.
Biochemistry 29:76
Rand, R. P. and Parsegian, V. A. BBA 988:351
d repeat
 
58
54.5
56.4
59.9
61.2
63.3
63.8
equilibrium
concentration
0.67
0. 
0.74
0. 
0.68
 
 
molecular
surface area
63.8
 
57.3
 
58.5
 
 
lipid bilayer
thickness
37.7
 
41.7
 
41.5
 
 
water layer
thickness
19.4
 
14.7
 
19.7
 
 
hydrocarbon
layer thickness
28.6
 
30.3
 
30.2
 
 
polar group
thickness
10.0
 
11.4
 
11.3
 
 
water volume
per lipid
619
 
421
 
576
 
 
lambda
 
1.8
 
1.3
 
2.1
 
 
Po (dyne/cm2)
 
10.2
 
11.2
 
10.0
 
 
G min (erg/cm2)
 
0.03
 
0.09
 
0.08
 
 
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Mixtures

Parameters

units of Angstroms unless otherwise indicated

DGDG/SOPC 44/55 DGDG/POPE 1/1 eggPC/chol 1/1 DPPC/chol 1/1 DPPC/chol 8/1 eggPC/DAG 12.5%
References
 
Lis, L. J. et.al. Biophys J. 37: 657and 667
Rand, R. P. et.al. Can. J. Biochem. 58:959
Rand, R. P. and Parsegian, V. A. BBA 988: 351
Das, S. and
Rand, R. P.
Biochem. Biophys. Res. Comm. 124: 491
d repeat
 
57.2
54
65.5
66
80
63
equilibrium
concentration
0.68
0.72
0.64
0.65
0.64
0.58
molecular
surface area
73
70
95.6
87.9
47.5
81.2
lipid bilayer
thickness
38.9
38.9
42
43.1
50.8
36.6
water layer
thickness
18.3
15.1
23.5
22.9
29.2
26.4
hydrocarbon
layer thickness
25.1
25.0
33.0
34.2
34.0
25.9
polar group
thickness
13.8
13.9
9.0
8.9
16.8
10.8
water volume
per lipid
665
530
1126
1005
694
1070
lambda
 
1.8
1.7
1.1
1.5
2.0
2.4
Po (dyne/cm2)
 
10.6
10.3
13.8
11.5
10.7
10.4
G min (erg/cm2)
 
0.18
0.23
0.003
0.01
0.004
0.05
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Hexagonal Phases

DOPE   with DOG with LysoPC's with LysoPE's with chol
no tetradecane   10.4% L-lysoPC L-lysoPE 10%
with tetradecane   14.8% O-lysoPC O-lysoPE 20%
    17.2% P-lysoPC S-lysoPE 30%
    25.8%     30% no tetradecane
          50% no tetradecane
DOPC with DCG with DOG with cholesterol    
  20% 4.8/1 33%    
  25% 4/1 43%    
  30% 3.16/1 50%    
  35% 2.5/1      
DPlPE          
DVPE          
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DOPE/DOG mixtures

Parameters

units of Angstroms unless otherwise indicated

DOPE DOPE/DOG 10.4% DOPE/DOG 14.8% DOPE/DOG 17.2% DOPE/DOG 25.8%
References
 
Gawrish, K.
et.al.
Biochemistry 31:2856
Leikin, S.
et.al
Biophys. J. 71:2623
d repeat
 
 
58.1
55.7
54.7
50.5
equilibrium
concentration
 
 
 
 
 
radius of
water cylinder
 
17.7
16.2
15.6
13.1
radius to
pivotal plane
 
 
 
 
 
dl interaxial 'bilayer'
thickness
 
31.7
31.8
31.9
32.1
dm interstitial lipid
thickness
 
42.1
41.8
41.7
41.2
interaxial hydrocarbon
thickness
 
20.6
20.7
20.7
20.9
interstitial hydrocarbon
thickness
 
31
30.6
30.5
29.9
area at the
water interface
 
52
52.9
53.6
55.4
area at the
pivotal plane
 
 
 
 
 
area at the
hydrocarbon tail
 
108.7
115.6
119.4
136.3
volume water
per lipid
 
460
430
419
362
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DOPE/lysoPC mixtures

Mixture DOPE/L-lysoPC DOPE/O-lysoPC DOPE/P-lysoPC
Parameters

units of Angstroms unless otherwise indicated

0% 4% 8% 12% 0% 2.5% 5.0% 7.5% 10.0% 0% 4% 8% 12%
References
 
                         
d repeat
 
68.5
70.6
75
79.7
68.5
71.8
72.8
76.1
79.1
71
71
76
79
equilibrium
concentration
0.713
0.710
0.693
0.673
0.719
0.696
0.690
0.668
0.651
0.706
0.716
0.700
0.685
radius of
water cylinder
21.9
22.7
24.8
27.2
21.6
23.6
24.2
26.1
27.9
22.9
22.5
24.8
26.4
radius to
pivotal plane
29.6
30.6
32.8
35.3
28.7
30.6
31.1
33.1
34.8
30.6
30.3
32.8
34.5
dl interaxial 'bilayer'
thickness
35.4
36.2
37.0
37.6
35.8
35.7
35.7
35.6
35.6
36.1
36.9
38.1
38.3
dm interstitial lipid
thickness
47.6
48.8
50.4
51.9
48.1
48.5
48.7
49.2
49.7
48.8
49.6
51.7
52.4
interaxial hydrocarbon
thickness
25.0
25.9
26.8
27.6
25.3
25.3
25.5
25.7
25.9
25.6
26.3
27.6
28.1
interstitial hydrocarbon
thickness
37.3
38.5
40.2
41.8
37.6
38.1
38.5
39.3
40.0
38.3
39.0
41.1
42.2
area at the
water interface
54.1
54.3
55.3
56.8
53.1
55.4
56.7
59.3
60.9
53.4
53.1
53.5
55.8
area at the
pivotal plane
73.2
73.3
73.2
73.8
70.5
71.8
73.1
75.0
76.0
71.1
71.4
70.8
72.7
area at the
hydrocarbon tail
107.9
107.7
106.5
105.9
107.1
107.3
108.7
109.9
110.1
105.1
106.3
104.3
106.0
volume water
per lipid
594
618
687
775
577
656
688
779
853
614
600
666
740
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DOPE/lysoPE mixtures

Mixture DOPE/L-lysoPE DOPE/O-lysoPE DOPE/S-lysoPE
Parameters

units of Angstroms unless otherwise indicated

0% 5% 10% 15% 0% 10% 15% 0% 3.9% 7.2% 9.7% 14.2%
References
 
                       
d repeat
 
71
72
76
80
68.5
73.5
75
68.5
69
71.5
72
76
equilibrium
concentration
0.724
0.717
0.689
0.663
0.717
0.683
0.673
0.729
0.726
0.709
0.706
0.680
radius of
water cylinder
22.2
22.8
25.3
27.7
21.7
24.7
25.6
21.2
21.5
23.0
23.3
25.6
radius to
pivotal plane
29.1
29.7
32.0
34.4
29.1
31.9
32.8
28.1
28.3
29.7
30.0
32.3
dl interaxial 'bilayer'
thickness
37.5
37.5
37.2
37.0
35.7
35.5
35.4
36.6
36.6
36.6
36.6
36.5
dm interstitial lipid
thickness
50.2
50.3
50.8
51.3
47.9
48.7
48.8
48.9
49.0
49.4
49.5
50.1
interaxial hydrocarbon
thickness
26.5
26.8
27.0
27.2
25.2
25.9
26.2
25.8
26.1
26.4
26.6
26.9
interstitial hydrocarbon
thickness
39.2
39.6
40.6
41.5
37.5
39.0
39.6
38.1
38.5
39.2
39.4
40.5
area at the
water interface
50.5
52.4
55.6
58.9
53.4
59.3
62.2
51.5
52.9
55.1
56.3
59.8
area at the
pivotal plane
66.2
68.1
70.4
73.1
71.6
76.7
79.7
68.0
69.7
71.4
72.6
75.3
area at the
hydrocarbon tail
102.7
105.1
106.4
108.4
107.4
112.4
116.1
105.7
108.1
109.2
110.9
112.8
volume water
per lipid
564
600
704
819
582
734
798
549
572
636
657
769
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DOPE/chol mixtures

Parameters

units of Angstroms unless otherwise indicated

DOPE/td DOPE/chol 10% DOPE/chol 20% DOPE/chol 30% DOPE notd DOPE/chol 30%no td DOPE/chol 50%no td
References
 
Chen, Z. and
Rand, R. P.
Biophys.J. 73:267
d repeat
 
68.5
66.6
64.8
62.7
65.0
64
63.5
equilibrium
concentration
0.700
0.715
0.73
0.75
0.687
0.692
0.699
radius of
water cylinder
22.4
21.2
20.1
18.7
22.0
21.6
21.2
radius to
pivotal plane
30.4
29.3
28.3
27.0
28.4
28.3
27.8
dl interaxial 'bilayer'
thickness
34.4
34.5
34.6
35.0
31.0
30.7
30.8
dm interstitial lipid
thickness
46.6
46.4
46.2
46.2
42.6
42.1
42.1
interaxial hydrocarbon
thickness
24.4
24.7
25.3
26.0
19.8
21.4
23.0
interstitial hydrocarbon
thickness
36.6
36.6
36.8
37.2
31.4
32.8
34.4
area at the
water interface
56.3
58.5
61.2
64.0
51.1
62.2
76.0
area at the
pivotal plane
76.7
80.9
86.1
92.5
65.9
81.4
99.2
area at the
hydrocarbon tail
109.9
116.9
125.6
136.5
95.8
117.2
144.4
volume water
per lipid
632
622
617
602
565
675
812
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DOPC/DAG mixtures

Mixture DOPC/DCG DOPC/DOG
Parameters

units of Angstroms unless otherwise indicated

20% 25% 30% 35% 17.2% 20% 24% 28.6%
References
 
               
d repeat
 
107
95
86.5
77
105.5
94.5
89.5
77.7
equilibrium
concentration
0.535
0.582
0.632
0.665
0.53
0.592
0.620
0.690
radius of
water cylinder
43.5
36.5
31.1
26.4
43.2
36.0
32.8
25.7
radius to
pivotal plane
48.5
41.6
37.2
32.3
49.2
42.3
39.3
32.4
dl interaxial 'bilayer'
thickness
36.5
36.6
37.6
36.1
35.4
37.1
37.7
38.4
dm interstitial lipid
thickness
55.6
53.6
53.1
49.9
54.2
54.0
53.7
52.2
interaxial hydrocarbon
thickness
26.2
26.6
27.6
26.8
25.5
27.0
27.7
28.5
interstitial hydrocarbon
thickness
45.3
43.6
43.1
40.6
44.4
43.9
43.7
42.4
area at the
water interface
70.0
71.4
70.8
75.6
73.4
70.4
71.6
70.7
area at the
pivotal plane
78.1
81.3
84.6
92.5
83.6
82.8
85.7
89.2
area at the
hydrocarbon tail
109.5
118.2
125.2
140.4
114.1
117.7
124.3
136.2
volume water
per lipid
1528
1310
1107
1001
1592
1272
1181
911
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DOPC/chol mixtures

Parameters

units of Angstroms unless otherwise indicated

DOPC/chol 33% DOPC/chol 43% DOPC/chol 50%
References
 
Chen, Z. and
Rand, R.P.
Biophys. J. 73:267
d repeat
 
112
102
94
equilibrium
concentration
0.51
0.56
0.61
radius of
water cylinder
47.1
40.7
35.3
radius to
pivotal plane
52.9
46.7
41.6
dl interaxial 'bilayer'
thickness
35.1
36.4
37.9
dm interstitial lipid
thickness
55.1
54.6
54.7
interaxial hydrocarbon
thickness
25.9
27.7
29.5
interstitial hydrocarbon
thickness
45.9
45.9
46.3
area at the
water interface
78.9
82.2
84.0
area at the
pivotal plane
88.5
94.3
98.9
area at the
hydrocarbon tail
119.3
131.2
142.4
volume water
per lipid
1866
1678
1488
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Phosphatidlyethanolamines

Parameters

units of Angstroms unless otherwise indicated

DOPE no td DOPE plus td DplPE no td DplPE plus td DVPE no td DVPE plus td
References
 
    Epand, R. M.
et.al.
Biophys. J. 71:1806
d repeat
 
64.5
67
lam
68.2
71
71.6
equilibrium
concentration
0.687
0.719
 
 
 
 
radius of
water cylinder
22.047
21.633
 
 
 
 
radius to
pivotal plane
28.448
28.699
 
 
 
 
dl interaxial 'bilayer'
thickness
30.962
35.832
 
 
 
 
dm interstitial lipid
thickness
42.573
48.068
 
 
 
 
interaxial hydrocarbon
thickness
19.821
25.335
 
 
 
 
interstitial hydrocarbon
thickness
31.432
37.572
 
 
 
 
area at the
water interface
51.054
53.114
 
 
 
 
area at the
pivotal plane
65.879
70.463
 
 
 
 
area at the
hydrocarbon tail
95.825
107.07
 
 
 
 
volume water
per lipid
565
577
 
 
 
 
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