Membrane
Biophysics
Laboratory

R. Peter Rand

* My CV

* Woodturning

* Osmotic Pressure Data

* Lipid Structural Data

Research Interests

My research interests are to understand the forces and energies that control the assembly and the conformation of biological molecules. This is motivated by the phenomena of membrane assembly, the fusion of membranes, molecular recognition and activity. The systems interogated, through the development of an osmotic stress method, are lipid bilayers and other selected molecular assemblies and single functioning molecules. The solvent, water, has been shown to contribute far more to the forces and energies of these molecular processes than heretofore recognized. The contribution of water binding reactions to the energetics of functioning enzyme activity through conformational change is being measured.

 

OSMOTIC STRESS

And Osmotic Pressure Measurements

Osmotic stress (OS) is an EXPERIMENTAL STRATEGY originally developed to measure hydration and other forces between phospholipid bilayers. It is now being used to study the energetics of other macromolecular assemblies and of single macromolecular conformational changes.

Some REFERENCES here describe the application of OS to membrane and macromolecular interactions, to membrane channel gating, and to small molecule binding and enzyme function.

An 'ART GALLERY' provides conceptual views of how it works in several specific applications. Here we are looking specifically at hexokinase and actin.

We provide here a DATA BASE of MEASURED OSMOTIC PRESSURES, seen below, of several solutes or osmolytes which can be used by those interested in doing osmotic stress experiments.

For more news on osmotic stress, and macromolecular forces check out the Laboratory of Structural Biology page at NIH in Washington



OS-niks


Here is a list of people,

who are either using osmotic stress experimentally, or have closely connected interests somehow.
- - - whom you can contact by e-mail by clicking on their name,
- - - or see what they are doing through their web site.


Department of Biological Sciences

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