Art van der Est

Professor of Chemistry

(Adjunct Professor of Physics)

Art van der Est

Office: CRN 408
Lab: CRN 130
Phone: 905 688 5550 x4602
Email: avde@brocku.ca

Research Interests

Photosynthesis; time resolved EPR spectroscopy; electron transfer reactions; light-induced electron spin polarization

Education

  • BSc (Hons) Chemistry (1979) University of British Columbia, supervisor: L.D. Hall
  • PhD Chemistry (1987) University of British Columbia, supervisor: E.E. Burnell
  • Habilitation Experimental Physics (1999) Freie Universität, Berlin, supervisor: D. Stehlik

Awards and Honours

  • Senior External Fellow, Freiburg Institute of Advanced Studies (2015)
  • Japan Society for the Promotion of Science Fellowship (2010)
  • Chancellor’s Chair Research Award (2003)
  • Premier’s Research Excellence Award (2000)

Our research focuses on using modern time-resolved electron spin resonance (ESR) spectroscopy to study the structure and function of photosynthetic reaction centres and porphyrin-based model systems. Light excitation of these systems leads to energy and electron transfer reactions involving short-lived paramagnetic intermediates. The spin polarized transient EPR spectra of these species can be used to deduce both structural as well as kinetic information about the functional states.
transient EPR dataset
Our interest in photosynthesis involves understanding how plants and bacteria capture and store sunlight. In particular, we are trying to elucidate the role that protein-cofactor interactions play in controlling the effeciency and rate of the transmembrane electron transfer in photosynthetic reaction centres. Transient ESR is especially well-suited for studying the quinone acceptors in these systems and has shown that protein-cofactor interactions have a dramatic influence on the properties of the quinones different in different reaction centres.

The work on porphyrin-based model systems is directed primarily towards mimicking the natural photosystems and creating complexes that are able to use visible light to oxidize water.  Together with Prof. Prashanth Poddutoori we have been studying complexes based on main group element porphyrins that are able to act as the photosensitizers in high potential photoanodes

  1. A. van der Est and P.K. Poddutoori (2017) “Time Resolved EPR in Artificial Photosynthesis” in “Photosynthesis: Structures, Mechanisms, and Applications” M.M. Najafpour, G. F. Moore, H.J.M. Hou and S.I. Allakhverdiev Eds (Springer, New York) Ch. 18, pp. 360-387.
  2. A. van der Est (2016) “Continuous-wave EPR” eMagRes, Vol 5: 1–11. S. Stoll and D. Goldfarb Eds. DOI 10.1002/9780470034590.emrstm1508
  3. “The Biophysics of Photosynthesis” J. Golbeck and A. van der Est Eds (2014) Springer (New York)
  4. J. Golbeck and A. van der Est (2013) “Electron Paramagnetic Resonance Spectroscopy” in “Molecular Biophysics for the Life Sciences” N. Allwell, L. Narhi and I. Rayment Eds (Springer, New York) Chapter 6, (175–213)
  5. A. van der Est and D. Stehlik (2009) “Transient EPR of Photosynthetic Electron Transfer Reactions” in “The Treasures of Eureka” Volume 1, K. Salikhov Ed “Electron Paramagnetic Resonance Spectroscopy: From Fundamental Research to Pioneering Applications & Zavoisky Award” AXAS Publishing Ltd Wellington, New Zealand.
  6. A. van der Est (2008) “Electron Transfer in Photosynthesis” in “The Wiley Encyclopedia of Chemical Biology” Wiley-Interscience (DOI: 10.1002/9780470048672.wecb450)
  7. “Photosynthesis: Fundamental Aspects to Global Perspectives” A. van der Est and D. Bruce Eds. (2005) Alliance Communications Group (Kansas City)
  8. A. van der Est (2006) “Electron Transfer Involving Phylloquinone in Photosystem I” in “Photosystem I: The Light-Driven, Plastocyanin:Ferredoxin Oxidoreductase” J. Golbeck Ed. Springer (Berlin/Heidelberg) Chapter 24, 387-411.
  9. K. Redding and A. van der Est (2006) “The Directionality of Electron Transfer in Photosystem I” in “Photosystem I: The Light-Driven, Plastocyanin:Ferredoxin Oxidoreductase” J. Golbeck Ed. Springer (Berlin/Heidelberg) Chapter 25, 412.
  10. van der Est, A. and Stehlik, D. (1995) Stuctural and Functional Properties of the State P+Q-, in Reaction Centers of Photosynthetic Bacteria (Michel-Beyerle, M. ed.), Springer Series in Biophysics, p. 321, Springer, Berlin.
  1. 2017
  1. G.N. Lim, S. Hedström, K.A. Jung, P.A. D. Smith, V.S. Batista, F. D’Souza, A. van der Est, and P.K. Poddutoori (2017) Interfacial Electron Transfer Followed by Photooxidation in N,N-Bis(p-anisole)aminopyridine–Aluminum(III) Porphyrin–Titanium(IV) Oxide Self-Assembled Photoanodes, J. Phys. Chem. C 121, 14484–14497
  2. B. Ferlez, A. Agostini, D. Carbonera, J.H. Golbeck, A. van der Est (2017) Triplet Charge Recombination in Heliobacterial Reaction Centers Does Not Produce a Spin-Polarized EPR Spectrum, Z. Phys. Chem. 231, 593-607
  3. P.K. Poddutoori, Y.E. Kandrashkin, A. van der Est (2017) A Transient EPR Study of Electron Transfer in Tetrathiafulvalene-Aluminum(III) Porphyrin-Anthraquinone Supramolecular Triads, Z. Phys. Chem. 231, 293-310

2016

  1. P.K. Poddutoori, G.N. Lim, M. Pilkington, F. D’Souza, A. van der Est (2016) Phosphorus(V) Porphyrin-Manganese(II) Terpyridine Conjugates: Synthesis, Spectroscopy and Photooxidation Studies on an SnO2; Surface Inorganic Chemistry 55, 11383–11395
  2. T. Chu, L. Belding, P.K. Poddutoori, A. van der Est, T. Dudding, I. Korobkov and G.I. Nikonov (2016) Unique Molecular Geometries of Reduced 4- and 5-Coordinate Zinc Complexes Stabilised by Diiminopyridine Ligand Dalton Trans. 45, 13440-13448
  3. Q. Fu, Z.R. Gray, A. van der Est, and R. H. Pelton (2016) Phase Behavior of Aqueous Poly(acrylic acid-g-TEMPO), Macromolecules 49, 4935–4939
  4. Yu. Kandrashkin, P. Poddutoori and A. van der Est (2016) Electron Transfer Pathways in a Tetrathiafulvalene-Aluminum(III) Porphyrin-Free-Base Porphyrin Triad Studied Using Electron Spin Polarization, Appl. Magn. Res. 47, 511-526
  5. C. Obondi, G.N. Lim, B. Churchill, P.K. Poddutoori, A. van der Est and F. D’Souza (2016) Modulating the generation of long-lived charge separated states exclusively from the triplet excited states in palladium porphyrin–fullerene conjugates, Nanoscale 8, 8333-8344.

2015

  1. T.L. Walker, S. Mula, W. Malasi, J.T. Engle, C.J. Ziegler, A. van der Est, J. Modarelli and M.J. Taschner (2015) Blue copper protein analogue: synthesis and characterization of copper complexes of the N2S2 macrocycle 1,8-dithia-4,11-diazacyclotetradecane, Dalton Trans. 44, 20200-20206.
  2. M. Gorka, A. Perez, C.S. Baker, B. Ferlez, A. van der Est, D.A. Bryant and J.H. Golbeck (2015) Electron Transfer from the A1A and A1B sites to a Tethered Pt Nanoparticle Requires the FeS Clusters for Suppression of the Recombination Channel, J. Photochem. Photobiol. B 152, 325-334.
  3. R. Pazdzior, Z. Yang, M.S. Mesbahuddin, J. Yee, A. van der Est, and S. Rafferty (2015) Low reduction potential cytochrome b5 isotypes of Giardia intestinalis Exp. Parasitol. 157, 197-201
  4. B. Ferlez, W. Dong, R. Siavashi, K. Redding, H. J. Hao, J.H. Golbeck and A. van der Est (2015) The Effect of Bacteriochlorophyll g Oxidation on Energy and Electron Transfer in Reaction Centers from Heliobacterium modesticaldum, J. Phys. Chem. B 119, 13714-13725
  5. P.K. Poddutoori, G.N. Lim, A.S. Sandanayaka, P.A. Karr, O. Ito, F. D’Souza, M. Pilkington, and A. van der Est (2015) Axially Assembled Photosynthetic Reaction Center Mimics Composed of Tetrathiafulvalene, Aluminum (III) Porphyrin and Fullerene Entities, Nanoscale 7, 12151-12165.
  6. P.K. Poddutoori, J.M. Thomsen, R.L. Milot, S.W. Sheehan, C.F. Negre, V.K. Garapati, C.A. Schmuttenmaer, V.S. Batista, G.W. Brudvig, A. van der Est, A. (2015). Interfacial electron transfer in photoanodes based on phosphorus (V) porphyrin sensitizers co-deposited on SnO2 with the Ir (III) Cp* water oxidation precatalyst, J. Mat. Chem. A. 3, 3868-3879.
  7. M.D. McConnell, J. Sun, R. Siavashi, A. Webber, K.E. Redding, J.H. Golbeck, J. H., A. van der Est, A. (2015). Species-dependent alteration of electron transfer in the early stages of charge stabilization in Photosystem I Biochim. Biophys. Acta, 1847, 429-440.

2014

  1. J. Sun, S. Hao, M. Radle, W. Xu, I. Shelaev, V. Nadtochenko, V. Shuvalov, A. Semenov, H. Gordon, A. van der Est and J. H. Golbeck (2014) Evidence that Histidine Forms a Coordination Bond to the A0A and A0B Chlorophylls and a Second H-Bond to the A1A and A1B Phylloquinones in M688HPsaA and M668HPsaB Variants of Synechocystis sp. PCC 6803, Biochim. Biophys. Acta 1837, 1362-1375
  2. M. Gorka, J. Schartner, S. Hilken, A. Berkessel, A. van der Est, M. Rögner and J. H. Golbeck (2014) Light-Mediated Hydrogen Generation in Photosystem I: Attachment of a Naphthoquinone– Molecular Wire–Pt Nanoparticle to the A1A and A1B Sites, Biochemistry 53,2295-2306.
  3. Yu. E. Kandrashkin and A. van der Est (2014) Orientation Information from the Dipolar Interaction Between a Complex in the Excited Quartet State and a Doublet Spin Label Appl. Magn. Reson. 45, 217-237
  4. T. Chu, L. Belding, A. van der Est, T. Dudding, I. Korobkov, and G.I. Nikonov (2014) A Coordination compound of Ge(0) stabilized by a diiminopyridine ligand Angew. Chem. 53, 2711– 2715

2013

  1. Mahboob A, Vassiliev S, Poddutoori PK, van der Est A, Bruce D (2013) Factors Controlling the Redox Potential of ZnCe6 in an Engineered Bacterioferritin Photochemical ‘Reaction Centre’. PLoS ONE 8(7): e68421.
  2. P.K. Poddutoori, N. Zarrabi, A.G. Moiseev, R. Gumbau-Brisa, S. Vasil’ev and A. van der Est (2013) Long-Lived Charge Separation in New Axial Donor-Porphyrin-Acceptor Triads Based on Tetrathiafulvalene, Aluminum(III) Porphyrin and Naphthalenediimide, Chem. Eur. J. 19, 3148-3161

2012

  1. S. Mula, M.D. McConnell, A. Ching, N. Zhao, H.L. Gordon, G. Hastings, K.E. Redding and A. van der Est (2012) Introduction of a Hydrogen Bond between Phylloquinone PhQA and a Threonine Side-chain OH Group in Photosystem I, J. Phys. Chem. B 116, 14008-14016
  2. A. van der Est and P.K. Poddutoori (2012) Light-Induced Spin Polarization in Porphyrin- Based Donor-Acceptor Dyads and Triads, Appl. Magn. Reson. 44, 301–318
  3. M.E. El-Khouly, A. Moiseev, A. van der Est, S. Fukuzumi, (2012) Photoinduced Electron Transfer in Zinc naphthalocyanine – Naphthalenediimide Supramolecular Dyads ChemPhysChem 13, 1191- 1198
  4. S. Mula, A. Savitsky, K. Möbius, W. Lubitz, J.H. Golbeck, M.D. Mamedov, A.Yu. Semenov, A. van der Est, (2012) Incorporation of a High Potential Quinone Reveals that Electron Transfer in Photosystem I Becomes Highly Asymmetric at Low Temperature Photochem. Photobiol. Sci. 11, 946-956
  5. A.G. Moiseev, P.K. Poddutoori and A. van der Est (2012) Spin Selective Electron Transfer and Charge Recombination in Self-Assembled Porphyrin Naphthalenediimide Dyads Appl. Magn. Res. 42, 41-55

2011

  1. N. Srinivasan, S. Santabarbara, F. Rappaport, D. Carbonera, K. Redding, A. van der Est and J.H. Golbeck (2011)  Alteration of the H-Bond to the A1A Phylloquinone in Photosystem I: Influence on the Kinetics and Energetics of Electron Transfer J. Phys. Chem. B 115, 1751-1759
  2. Yu. Kandrashkin and A. van der Est (2011) Stimulated Electron Spin Polarization in Strongly Coupled Triplet-Doublet Spin Pairs Appl. Magn. Reson. 40,189-204
  3. P.K. Poddutoori, A.S.D. Sandanayaka, N. Zarrabi, T. Hasobe, O. Ito, A. van der Est (2011) Sequential Charge Separation in Two Axially Linked Phenothiazine-Aluminum(III) Porphyrin-Fullerene Triads J. Phys. Chem. A 115, 709-717
  4. D.V. Gutsulyak, A. van der Est and G.I. Nikonov (2011)  Facile Catalytic Hydrosilylation of Pyridines Angew. Chem. 50, 1384-1387
  5. D.M. Spasyuk, S.I. Gorelsky, A. van der Est and D. Zargarian (2011) Characterization of Divalent and Trivalent Species Generated in the Chemical and Electrochemical Oxidation of a Dimeric Pincer Complex of Nickel Inorg. Chem. 50, 2661-2674

2000-2010

  1. P.K. Poddutoori, A. Dion, S. Yang, M. Pilkington, J.D. Wallis, A. van der Est (2010)  Light-induced hole transfer in a hypervalent phosphorus(V) octaethylporphyrin bearing an axially linked bis(ethylenedithio)tetrathiafulvalene J. Porph. Phthal.  14, 178-187
  2. P.K. Poddutoori, A.S. D. Sandanayaka, T. Hasobe, O. Ito, A. van der Est (2010)  Photoinduced Charge Separation in a Ferrocene-Aluminum(III) Porphyrin-Fullerene Supramolecular Triad J. Phys. Chem B  114, 14348-14357
  3. P.K. Poddutoori, M. Pilkington, A. Alberola, V. Polo, J.E. Warren, A. van der Est (2010) Spin-Spin Interactions in Porphyrin-Based Monoverdazyl Radical Hybrid Spin Systems Inorg. Chem.  49 3516-3524
  4. A. van der Est, S. Chirico, I. Karyagina, R. Cohen, G. Shen, J. H. Golbeck (2010) Alteration of the Axial Met Ligand to Electron Acceptor A0 in Photosystem I: An Investigation of Electron Transfer at Different Temperatures by Multifrequency Time Resolved and CW EPR Appl. Magn. Res.  37 103-121
  5. A. van der Est, Yu. Pushkar, I. Karyagina, B. Fonovic, T. Dudding, J. Niklas, W. Lubitz and J.H. Golbeck (2010)  Incorporation of 2,3-Disubstituted-1,4-naphthoquinones into the A1 Binding Site of Photosystem I Studied by EPR and ENDOR Spectroscopy Appl. Magn. Res. 37 65-83
  6. D. Spasyuk, D. Zargarian, A. van der Est (2009)  New POCN-Type Pincer Complexes of Ni(II) and Ni(III) Organometallics  28 6531-6540
  7. N. Srinivasan, I. Karyagina, R. Bittl, A. van der Est, and J.H. Golbeck (2009) Role of the Hydrogen Bond from Leu722 to the A1A Phylloquinone in Photosystem I Biochemistry  48 3315-3324
  8. A. van der Est (2009) Transient EPR: Using Spin Polarization in Sequential Radical Pairs to Study Electron Transfer in Photosynthesis Photosynth. Res. 102 335-347
  9. S. Li, A. van der Est, N. Bunce (2009)  Electrochemical oxidation of oxalate ion in the presence of fluoride ion, and radical analysis by ESR Electrochim. Acta 54 3589-3593
  10. R. Agalarov, M. Byrdin, F. Rappaport, G. Shen, D. Bryant, A. van der Est and J.H. Golbeck (2008) Removal of the PsaF Polypeptide Biases Electron Transfer in Favor of the PsaB Branch of Cofactors in Triton X-100 Photosystem I Complexes from Synechococcus sp. PCC 7002 Photochem. Photobiol. 84 1371-1380
  11. P.K. Poddutoori, P. Poddutoori, B.G. Maiya, T.K. Prasad, Yu. Kandrashkin, S. Vasil’ev, D. Bruce and A. van der Est (2008)  Redox control of photo-induced electron transfer in axial terpyridoxy porphyrin complexes Inorg. Chem. 47 7512-7522
  12. I. Karyagina, Yu. Pushkar, D. Stehlik, A. van der Est, H. Ishikita, E. Knapp, B. Jagannathan, R. Agalarov, and J. Golbeck (2007)  Contributions of the Distant Protein Environment to the Redox Potentials of the Electron Acceptor A1 Phylloquinones and the FX Iron-Sulfur Cluster in Photosystem I Biochemistry 46 10804-10816
  13. M. Chahma, K. Macnamara, A. van der Est, A. Alberola, V. Polo and M. Pilkington(2007) Synthesis and characterization of a TTF–verdazyl radical; a new building block for conducting and/or magnetic systems New J. Chem. 31, 1973-1978.
  14. Yu. E. Kandrashkin and A. van der Est(2007) Time Resolved EPR of Photosynthetic Reaction Centres: from Theory to Experiment Appl. Magn. Res.31, 105-122.
  15. Yu. E. Kandrashkin, M. S. Asano and A. van der Est(2006) Light-induced Electron Spin Polarization in Vanadyl Octaethylporphyrin: I. Characterization of the Excited Quartet State J. Phys. Chem. A. 110, 9607-9616.
  16. Yu. E. Kandrashkin, M. S. Asano and A. van der Est (2006) Light-induced Electron Spin Polarization in Vanadyl Octaethylporphyrin: II Dynamics of the Excited States J. Phys. Chem. A. 110, 9617-9626
  17. Y. Li, A. van der Est, M.G. Lucas, V. M. Ramesh, F. Gu, A. Petrenko, S. Lin, A.N. Webber, F. Rappaport, and K. Redding (2006) Directing electron transfer within Photosystem I by breaking H-bonds in the cofactor branches Proc. Natl. Acad. Sci. USA 103, 2144-2149.
  18. M. Chahma, M. Pilkington, X. Wang and A. van der Est (2006) Synthesis and Characterization of a New Family of Spin Bearing TTF Ligands J. Org. Chem.  71, 2750-2755
  19. Yu. E. Kandrashkin, M. S. Asano and A. van der Est (2006) Observation of a photoexcited state of a paramagnetic transition metal complex by time-resolved electron paramagnetic resonance spectroscopy Phys. Chem. Chem. Phys.  8, 2129-2132.
  20. Yu. N. Pushkar, I. Karyagina, D. Stehlik, S. Brown and A. van der Est (2005) Recruitment of a Foreign Quinone into the A1 Site of Photosystem I: Consecutive Forward Electron Transfer From A0 to A1 to Fx with Anthraquinone in the A1 site as studied by transient EPR. J. Biol. Chem. 280, 12382-12390.
  21. Yu. Kandrashkin and A. van der Est (2004) Electron spin polarization of the excited quartet state of strongly coupled triplet–doublet spin systems J. Chem. Phys. 120, 4790-4799
  22. R. Cohen, G. Shen, J. H. Golbeck, W. Xu, P. Chitnis, A. Valieva, A. van der Est, J. Pushkar, D. Stehlik and P. Steinbach (2004) Evidence for Asymmetric Electron Transfer in Cyanobacterial Photosystem I: Analysis of a Methionine-to-Leucine Mutation of the Ligand to the Primary Electron Acceptor A0. Biochemistry 43, 4741-4754
  23. A. van der Est, A. Valieva, Yu. E. Kandrashkin, G. Shen, D. Bryant and J. Golbeck  (2004)  Removal of PsaF Alters Forward Electron Transfer in Photosystem I: Evidence for Fast Reoxidation of QK-A in Subunit Deletion Mutants of Synechococcus sp. PCC 7002 Biochemistry 43, 1264-1275
  24. V. Rozenshtein, A. Berg, H. Levanon, U. Krueger, D. Stehlik, Y. Kandrashkin and A. van der Est (2003) Observation of Photoinduced Electron Spin Polarization in the Ground State of Copper Porphyrins Israel J. Chem 43, 373-381.
  25. Yu. Kandrashkin and A. van der Est, (2003) Light-Induced Electron Spin Polarization in Rigidly Linked, Strongly Coupled Triplet-Doublet Spin Pairs Chem. Phys. Lett. 379 574-580
  26. Xu, W., Chitnis, P., Valieva, A., van der Est, A., Pushkar, J., Krzystyniak, M., Teutloff, C.,  Zech, S., Bittl, R., Stehlik, D., Zybailov, B., Shen, G., Golbeck, J. (2003) Electron Transfer in Cyanobacterial Photosystem I. I. Physiological and Spectroscopic Characterization of Site-Directed Mutants in a Putative Electron Transfer Pathway from A0 through A1 to FX, J. Biol. Chem. 278, 27876 – 27887
  27. Xu, W., Chitnis, P., Valieva, A., van der Est, A., Brettel, K., Guergova-Kuras, M., Pushkar, J., Zech, S.,  Stehlik, D., Shen, G., Zybailov, B., Golbeck, J. (2003) Electron Transfer in Cyano-bacterial Photosystem I.II Determination of Forward Electron Transfer Rates of Site-Directed Mutants in a Putative Electron Transfer Pathway from A0 through A1 to FX, J. Biol Chem. 278, 27864 – 27875
  28. J. Pushkar, S.G. Zech, D. Stehlik, S. Brown and A.van der Est, H. Zimmermann (2002) Orientation and protein-cofactor interactions of monosubstituted n-alkyl naphthoquinones in the A1 binding site of Photosystem I,  J. Phys. Chem B 106, 12052-12058.
  29. A. van der Est, M. Asano-Someda, P. Ragogna, Y. Kaizu (2002) Light-induced Electron Spin Polarization of a Weakly Coupled Triplet-Doublet Spin Pair in a Covalently Linked Porphyrin Dimer, J. Phys. Chem. A 106,8531-8542.
  30. Shen, G. Antonkine, M., van der Est, A., Vassiliev, I., Stehlik, D., Golbeck, J., and Bryant, D.(2002) Cloning and Mutagenesis of a New Gene Encoding a Rubredoxing-s Protein in the Cyanobacterium Synechococcus sp. PCC 7002: A Possible Function in the Assembly of three Fe-S Clusters in Photosystem I. J. Biol. Chem 277 20355-20366
  31. Kandrashkin, Yu., Vollmann, W., Stehlik, D., Salikhov, K., and van der Est, A. (2002) The Magnetic Field Dependence of the Electron Spin Polarization in Consecutive Spin-Correlated Radical Pairs in Type I Photosynthetic Reaction Centres  Mol. Phys. 100,  1431 – 1443
  32. Sakuagi, Y, Zybailov, B., Shen, G., Chitnis, P., van der Est, A., Bittl, R., Zech, S., Stehlik, D., Golbeck, J. and Bryant, D.(2002) Interruption of the menG Gene in the Phylloquinone Biosynthetic Pathway of Synechocystis sp. PCC 6803. Alteration of the Equilibrium Constant between A1 and FX by Incorporation of 2-Phytyl-1,4-Naphthoquinone into Photosystem I Biochemistry 41(1); 394-405.
  33. Kandrashkin, Yu. and van der Est, A., (2002) Electron Spin Polarization in Photosynthetic Reaction Centres: Strategies for Extracting Structural and Functional Information, RIKEN Review, 44, 124-127.
  34. van der Est, A. (2001) Light-Induced Spin Polarization in Type I Photosynthetic Reaction Centres Biochim. Biophys. Acta 1507, 212-225.
  35. Kandrashkin, Yu., and van der Est, A. (2001) A New Approach to Determining the Geometry of Weakly Coupled Radical Pairs from their Electron Spin Polarization Patterns Spectrochim. Acta A57, 1697-1709.
  36. Semenov, A., Vassiliev, I., van der Est, A., Mamedov, M., Zybailov, B., Shen, G., Stehlik, D., Diner, B., Chitnis, P., and Golbeck, J. (2000) Recruitment of a Foreign Quinone into the A1 Site of Photosystem I. III. Altered Electron Transfer Kinetics in Phylloquinone Biosynthetic Pathway Mutants Studied by Time Resolved Optical, EPR, and Electrometric Techniques J. Biol. Chem. 27523429-23438.
  37. Zybailov, B., van der Est, A., Zech, S., Teutloff, C., Johnson, W., Shen, G., Bittl, R., Stehlik, D., Chitnis, P., and Golbeck, J. (2000) Recruitment of a Foreign Quinone into the A1 Site of Photosystem I. II. Structural and Functional Characterization of Phylloquinone Biosynthetic Pathway Mutants by EPR and ENDOR Spectroscopy J. Biol. Chem. 275, 8531-8539.

1990-1999

  1. Asano-Someda, M., van der Est, A., Krueger, U., Stehlik, D., Kaizu, Y., and Levanon, H., (1999) Intramolecular Energy Transfer in a Covalently Linked Copper (II) Porphyrin – Free Base Porphyrin Dimer: Novel Spin Polarization in the Energy Acceptor, J. Phys. Chem. 103, 6704–6714.
  2. Salikhov, K., van der Est, A., and Stehlik, D. (1999) Spin Dynamics and EPR Spectral Simulations of Three-Spin Systems in Photosynthetic Reaction Centres, Appl. Magn. Res 16,101-134.
  3. van der Est, A., Hager-Braun, C., Leibl, W., Hauska, G., and Stehlik, D. (1998) Transient EPR Spectroscopy on Green-Sulfur Bacteria and Heliobacteria at two Microwave Frequencies, Biochim. Biophys. Acta 1409, 87–98.
  4. Kandrashkin, Y., Salikhov, K., van der Est, A., and Stehlik, D. (1998) Electron Spin Polarization in Consecutive Spin-Correlated Radical Pairs: Application to Short-Lived and Long-Lived Precursors in Type 1 Photosynthetic Reaction Centres Appl. Magn. Res. 15, 417–447.
  5. Kamlowski, A., Altenberg-Greulich, B., van der Est, A., Zech, S., Bittl, R., Fromme, P., Lubitz, W., and Stehlik, D.  (1998) The Quinone Acceptor A1 in Photosystem I: Binding Site, and Comparison to QA in Purple Bacteria Reaction Centers, J. Phys. Chem B 102, 8278-8287.
  6. van der Est, A., Reiss-Husson, F., and Stehlik, D. (1998) An investigation of the light-induced spin polarization in reaction centres of Rhodobacter sphaeroides Y, Photosynth. Res. 55, 217–225.
  7. Zech, S., van der Est, A.J., and Bittl, R. (1997) Measurement of Cofactor Distances Between P700+ and A1- in Native and Quinone Substituted Photosystem I using Pulsed EPR Spectroscopy, Biochemistry 36, 9774–9779.
  8. van der Est, A., Prisner, T., Bittl, R., Fromme, P., Lubitz, W., Moebius, K., and Stehlik, D. (1997) Time resolved X-, K-, and W-band EPR of the radical pair state P+A1- of Photosystem I in comparison with P+QA- in bacterial reaction centres, J. Phys. Chem. B 101, 1437-1443.
  9. van der Est, A., Fuechsle, G., Stehlik, D., and Wasielewski, M. (1997) Separation of the exchange and dipolar contributions to the spin-spin coupling in the donor-acceptor complex TAPD-ZnP-NQ, Appl. Mag. Res. 13, 317–335.
  10. Kamlowski, A., van der Est, A., Fromme, P., Krauss, N., Schubert, W.-D., Klukas, O., and Stehlik, D. (1997) The Structural Organisation of the PsaC Protein in Photosystem I from Single Crystal EPR and X-Ray Crystallographic Studies, Biochim. Biophys. Acta 1319, 199-213.
  11. Kamlowski, A., van der Est, A., Fromme, P., and Stehlik, D. (1997) Low temperature EPR on Photosystem I Single Crystals: Orientation of the Iron-Sulfur Centers FA and FB, Biochim. Biophys. Acta 1319, 185-198.
  12. Rendell, J., Zimmerman, D., van der Est, A.J., and Burnell, E. (1997) Orientational Order of 1,3-dichloro-2-ethenylbenzene in Liquid Crystal Solvents, Can. J. Chem. 75, 1156–1161.
  13. Kamlowski, A., Frankemoeller, L., van der Est, A., Stehlik, D., and Holzwarth, A. (1996) Evidence for the Delocalization of the Tiplet State 3P680 in the D1D2cytb559-Complex of Photosystem II, Ber. der  Bunsenges. Phys. Chem. 100, 2045–2051.
  14. van der Est, A., Fuechsle, G., Stehlik, D., and Wasielewski, M. (1996) X- and K-band Transient EPR of the Light Induced Radical Ion Pairs in Photosynthetic Model Systems, Ber. Bunsenges. Phys. Chem. 100, 2081–2085.
  15. Stehlik, D., van der Est, A., and Kamlowski, A. (1996) Time Resolved EPR Studies of Light-Induced Charge Separation in Photosynthetic Reaction Centers, Mol. Phys. Rep. 13, 21–36.
  16. Stehlik, D., van der Est, A., and Kamlowski, A. (1996) Transient Spin States in the Primary Processes of Photosynthesis, Ber. Bunsenges. Phys. Chem. 100, 2028–2035.
  17. Hugerat, M., van der Est, A., Ojadi, E., Bicczok, L., Linschitz, H., Levanon, H., and Stehlik, D. (1996) Transient EPR Studies of Ion-Paired Metallo-Porphyrin Heterodimers, J. Phys. Chem 100, 495–500.
  18. van der Est, A., Sieckmann, I., Lubitz, W., and Stehlik, D. (1995) Differences in the binding of the primary quinone acceptor in Photosystem I and reaction centres of Rhodobacter sphaeroides R-26 studied with transient EPR spectroscopy., Chem. Phys. 194, 349–359.
  19. Prisner, T., van der Est, A., Bittl, R., Lubitz, W., Stehlik, D., and Moebius, K. (1995) Time-resolved W-band (95 GHz) EPR spectroscopy of Zn-substituted reaction centers of Rhodobacter sphaeroides R-26., Chem. Phys. 194, 361–370.
    Bittl, R., van der Est, A., Kamlowski, A., Lubitz, W., and Stehlik, D. (1994) Time-resolved EPR of the radical pair P865+QA- in bacterial reaction centers. Observation of transient nutations, quantum beats and envelope modulations, Chem. Phys. Lett. 226, 349–358.
  20. van der Est, A., Bock, C., Golbeck, J., Brettel, K., Setif, P., and Stehlik, D. (1994) Electron transfer from the acceptor A1 to the iron-sulfur centres in photosystem I as studied by transient EPR spectroscopy, Biochemistry 33, 11789-11797.
  21. van der Est, A., Bittl, R., Abresch, E., Lubitz, W., and Stehlik, D. (1993) Transient EPR Spectroscopy of Perdeuterated Zn-Substituted Reaction Centres of Rhodobacter sphaeroides R-26, Chem. Phys. Lett. 212, 561–568.
  22. Fuechsle, G., Bittl, R., van der Est, A., Lubitz, W., and Stehlik, D. (1993) Transient EPR Spectroscopy of the Charge Separated State +Q- in Photosynthetic Reaction Centers. Comparison of Zn-substituted Rhodobacter sphaeroides R-26 and photosystem I, Biochim. Biophys. Acta 1142, 23–35.
  23. Sieckmann, I., Brettel, K., Bock, C., van der Est, A., and Stehlik, D. (1993) Transient Electron Paramagnetic Resonance of the Triplet State of P700 in Photosystem I: Evidence for Triplet Delocalization at Room Temperature, Biochemistry 32, 4842–4847.
  24. Brettel, K., Sieckmann, I., Fromme, P., van der Est,  A.., and  Stehlik, D. (1992) Low Temperature EPR on Single Crystals of Photosystem I, Study of the Iron-Sulfur-Center FA.,  Biochim. Biophys. Acta 1098, 266–270.
  25. Sieckmann, I.,  van der Est,  A.., Bottin, H., Setif, P., and  Stehlik, D. (1991) Nanosecond Electron Transfer Kinetics in Photosystem I  Following Substitution of Quinones for Vitamin K1 as Studied by Time  Resolved EPR,  FEBS Lett. 284, 98–102.
  26. Gierer, M.,  van der Est,  A.., and Stehlik, D. (1991) Transient  EPR of Weakly Coupled Spin-Correlated Radical Pairs in Photosynthetic  Reaction Centres: Increased Spectral Resolution from Nutation Analysis,  Chem. Phys. Lett. 186, 238–247.
  27. Schulz, M.,  van der Est,  A.., Rössler, E., and Vieth, H.-M.  (1991) 2H NMR Study of Benzene-d6 in a Liquid-Crystalline Polysiloxane,  Macromolecules 24, 5040–5045.

1980-1989

  1. Bock, C.,  van der Est,  A.J.., Brettel, K., and Stehlik, D. (1989)  Nanosecond Electron Transfer Kinetics in Photosystem I as Obtained from  Transient EPR at Room Temperature,  FEBS Lett. 247, 91–96.
  2. van der Est,  A.J.., Burnell, E., and Lounila, J. (1988) Molecular  Hydrogen in Nematic Liquid Crystals: Unusual Isotope Effects,  J. Chem. Soc. Faraday Trans. 84, 1095–1108.
  3. van der Est,  A.J.., Burnell, E., Barnhoorn, J., de Lange, C., and  Snijders, J. (1988) Acetylene in Nematic Liquid Crystals: A Vibrational  Analysis of the Observed Dipolar Couplings,  J. Chem. Phys. 89, 4657–4665.
  4. Hoatson, G., Bailey, A., van der Est,  A.J., Bates, G., and  Burnell, E. (1988) 2H-N.M.R. Studies of Flexibility and Orientation Order  in the Nematic Liquid Crystals,  Liquid Crystals 3, 683–694.
  5. Kok, M., van der Est,  A.J., and Burnell, E. (1988) Orientation  Order of Biaxial Solutes in the Nematic Phases: Size and Shape Effects,  Liquid Crystals 3, 485–505.
  6. Burnell, E.,  van der Est,  A.J., Patey, G., de Lange, C., and  Snijders, J. (1987) NMR of Solutes in Liquid Crystalline Solvents: Mechanisms  of Orientational Ordering,  Bull. Mag. Res. 9, 4–19.
  7. Weaver, A.,  van der Est,  A.J.., Rendell, J., Hoatson, G., Bates,  G., and Burnell, E. (1987) Orientation Mechanisms for Small Solutes in the  Nematic Liquid Crystal 5CB-ab-d4, Liquid Crystals 2, 633–642.
  8. van der Est,  A.J.., Kok, M., and Burnell, E. (1987) Size and Shape  Effects on the Orientation of Rigid Molecules in Nematic Liquid Crystals,  Mol. Phys. 60, 397–413.
  9. van der Est,  A.J., Barker, P., Burnell, E., de Lange, C., and  Snijders, J. (1985) NMR of Methane in Liquid Crystal Mixtures,  Mol. Phys. 56, 161.
  10. Barker, P.,  van der Est,  A.J., Burnell, E., Patey, G., deLange,  C., and Snijders, J. (1984) NMR of Deuterium in Liquid Crystal Mixtures,  Chem. Phys. Lett. 107, 426–430.

Courses for the 2017/2018 academic year:

CHEM 4P67 Biophysical Techniques (also offered as BTEC 4P67 and BCHM 4P67)

CHEM 3P53 Atomic and Molecular Structure and Spectroscopy

CHEM 5P13 Introduction Electron Spin Resonance Spectroscopy

Other courses:

CHEM 2P12 Introduction to Modern Physical Chemistry

CHEM 4P17 Biophysical Photochemistry

CHEM 1P91 Chemical Principles and Properties I